Journal: BMC Cardiovascular Disorders

Article Title: Circular RNA expression profile and its potential regulative role in human abdominal aortic aneurysm

doi: 10.1186/s12872-020-01374-8

Figure Lengend Snippet: circRNAs expression profiles detected by microarray in the AAA group and control group. a The box plot shows the nearly identical distributions of normalized intensity values from the aortic samples of the AAA and control group. b The scatter plot is built to assess the expression variation of circRNAs between the two groups. The X and Y axes indicate the normalized intensity values of each circRNAs from the AAA and control group. The dots above the upper green line and below the lower green line represent the dysregulated circRNAs with a fold change (FC) > 2.0 between the two groups. c The volcano plot presents differentially expressed circRNAs in AAA. The vertical lines correspond to 2-fold upregulation and downregulation, the horizontal line indicates P value of 0.05. The red dots represent the differentially expressed circRNAs (FC > 2.0 and P value < 0.05). d Hierarchical clustering analysis reveals a distinguishable expression profile of circRNAs between the AAA and control group. Each column indicates an aortic sample, each row represents a circRNA. The red and green color indicate high and low expression level, respectively. e Chromosomal distribution of the differentially expressed circRNAs between the two groups

Article Snippet: The fragmented labeled cRNAs were hybridized onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2).

Techniques: Expressing, Microarray, Control

Journal: BMC Cardiovascular Disorders

Article Title: Circular RNA expression profile and its potential regulative role in human abdominal aortic aneurysm

doi: 10.1186/s12872-020-01374-8

Figure Lengend Snippet: Validation of six randomly selected dysregulated circRNAs by qRT-PCR. Each circRNA was evaluated at least three times and compared with the results of microarray. The Y axis indicates the fold change of AAA vs control of each circRNA

Article Snippet: The fragmented labeled cRNAs were hybridized onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2).

Techniques: Biomarker Discovery, Quantitative RT-PCR, Microarray, Control

Journal: BMC Cardiovascular Disorders

Article Title: Circular RNA expression profile and its potential regulative role in human abdominal aortic aneurysm

doi: 10.1186/s12872-020-01374-8

Figure Lengend Snippet: The predicted circRNA/miRNA interaction networks for six randomly selected circRNAs. a , b The red nodes indicate upregulated circRNAs. c - f The blue nodes represent downregulated circRNAs. The green nodes are five complementary binding miRNAs of each circRNA

Article Snippet: The fragmented labeled cRNAs were hybridized onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2).

Techniques: Binding Assay

Journal: Frontiers in Genetics

Article Title: Systematic Identification of circRNA–miRNA–mRNA Regulatory Network in Esophageal Squamous Cell Carcinoma

doi: 10.3389/fgene.2021.580390

Figure Lengend Snippet: Differentially expressed circRNAs in the plasma of ESCC patients.

Article Snippet: The resulting hybridization solution (50 μl) was placed in a gasket slide, which was then assembled with the Arraystar Human CircRNA Microarray Slides V2.0 (8 × 15K; Arraystar), with a total of 13,617 circRNA probes on the microarray, according to the kit’s instructions.

Techniques: Clinical Proteomics

Journal: Frontiers in Genetics

Article Title: Systematic Identification of circRNA–miRNA–mRNA Regulatory Network in Esophageal Squamous Cell Carcinoma

doi: 10.3389/fgene.2021.580390

Figure Lengend Snippet: Differentially expressed circRNAs in ESCC patients. (A) The scatter plot of differentially expressed circRNAs. Green lines represent fold change of 2.0. (B) Volcano plot of differentially expressed circRNAs. The green vertical lines correspond to a fold change of 2.0, and the green horizontal line corresponds to a P -value of 0.05. (C) Hierarchical clustering of differentially expressed circRNAs. Each column represents a sample and each row represents a circRNA. The color reflects the expression level of circRNAs and changes from red (high) to black (medium) to green (low). (D) Bar plot shows the distributions of the differentially expressed circRNAs in human chromosomes.

Article Snippet: The resulting hybridization solution (50 μl) was placed in a gasket slide, which was then assembled with the Arraystar Human CircRNA Microarray Slides V2.0 (8 × 15K; Arraystar), with a total of 13,617 circRNA probes on the microarray, according to the kit’s instructions.

Techniques: Expressing

Journal: Frontiers in Genetics

Article Title: Systematic Identification of circRNA–miRNA–mRNA Regulatory Network in Esophageal Squamous Cell Carcinoma

doi: 10.3389/fgene.2021.580390

Figure Lengend Snippet: CircRNA–miRNA–mRNA interaction network in ESCC. CircRNAs are represented by diamonds; miRNAs are shown as triangles; the DEGs are represented by ellipses. Red represents upregulated expression, and blue color represents downregulated expression. circRNA, circular RNA; miRNA, microRNA; ESCC, esophageal squamous cell carcinoma; DEGs, differentially expressed genes.

Article Snippet: The resulting hybridization solution (50 μl) was placed in a gasket slide, which was then assembled with the Arraystar Human CircRNA Microarray Slides V2.0 (8 × 15K; Arraystar), with a total of 13,617 circRNA probes on the microarray, according to the kit’s instructions.

Techniques: Expressing

Journal: Frontiers in Genetics

Article Title: Systematic Identification of circRNA–miRNA–mRNA Regulatory Network in Esophageal Squamous Cell Carcinoma

doi: 10.3389/fgene.2021.580390

Figure Lengend Snippet: Sub-network of circRNAs, miRNAs, and hub genes. circRNAs are represented by diamonds; miRNAs are shown as triangles; hub genes are represented by ellipses. Red represents upregulated expression, and blue color represents downregulated expression. circRNA, circular RNA; miRNA, microRNA.

Article Snippet: The resulting hybridization solution (50 μl) was placed in a gasket slide, which was then assembled with the Arraystar Human CircRNA Microarray Slides V2.0 (8 × 15K; Arraystar), with a total of 13,617 circRNA probes on the microarray, according to the kit’s instructions.

Techniques: Expressing

Journal: The Journal of International Medical Research

Article Title: Potential mechanism of circRNA_000585 in cholangiocarcinoma

doi: 10.1177/03000605211024501

Figure Lengend Snippet: Primer details for quantitative real-time reverse transcription–polymerase chain reaction of circRNA_000585.

Article Snippet: The labelled cRNA mixture was diluted by adding 25 μl of 2× hybridization buffer, and 50 μl of the hybridization solution was transferred into the gasket slide, which was then assembled onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2 [8 × 15 K]).

Techniques: Reverse Transcription Polymerase Chain Reaction, Sequencing, Amplification

Journal: The Journal of International Medical Research

Article Title: Potential mechanism of circRNA_000585 in cholangiocarcinoma

doi: 10.1177/03000605211024501

Figure Lengend Snippet: Heatmap of circular (circ)RNA expression levels in cholangiocarcinoma (CCA) tumour specimens (P1–3) and matched para-cancer specimens (C1–3) from three patients with CCA. Each block represents different circRNA expression levels (red represents high expression; green represents low expression).

Article Snippet: The labelled cRNA mixture was diluted by adding 25 μl of 2× hybridization buffer, and 50 μl of the hybridization solution was transferred into the gasket slide, which was then assembled onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2 [8 × 15 K]).

Techniques: RNA Expression, Blocking Assay, Expressing

Journal: The Journal of International Medical Research

Article Title: Potential mechanism of circRNA_000585 in cholangiocarcinoma

doi: 10.1177/03000605211024501

Figure Lengend Snippet: Fold change in circular (circ)RNA expression levels in cholangiocarcinoma (CCA) tumour specimens and matched para-cancer specimens from three patients with CCA. (A) scatter plot (dots above the upper line represent fold-change >1.5, dots below the lower line represent fold-change >–1.5); and (B) volcano plot of circRNA expression (red dots represent fold-change >1.5 or >–1.5).

Article Snippet: The labelled cRNA mixture was diluted by adding 25 μl of 2× hybridization buffer, and 50 μl of the hybridization solution was transferred into the gasket slide, which was then assembled onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2 [8 × 15 K]).

Techniques: RNA Expression, Expressing

Journal: The Journal of International Medical Research

Article Title: Potential mechanism of circRNA_000585 in cholangiocarcinoma

doi: 10.1177/03000605211024501

Figure Lengend Snippet: Vertical scatter plot showing elevated circRNA_000585 expression in cholangiocarcinoma (CCA) tumour tissue versus matched para-cancer tissue from 15 patients with CCA (central horizontal line represents mean, upper and lower horizonal lines represent SD; P = 0.003 between groups).

Article Snippet: The labelled cRNA mixture was diluted by adding 25 μl of 2× hybridization buffer, and 50 μl of the hybridization solution was transferred into the gasket slide, which was then assembled onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2 [8 × 15 K]).

Techniques: Expressing

Journal: The Journal of International Medical Research

Article Title: Potential mechanism of circRNA_000585 in cholangiocarcinoma

doi: 10.1177/03000605211024501

Figure Lengend Snippet: Association between circRNA_000585 expression and clinicopathological characteristics in 15 patients with cholangiocarcinoma.

Article Snippet: The labelled cRNA mixture was diluted by adding 25 μl of 2× hybridization buffer, and 50 μl of the hybridization solution was transferred into the gasket slide, which was then assembled onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2 [8 × 15 K]).

Techniques: Expressing

Journal: Frontiers in Molecular Neuroscience

Article Title: Differential Expression Profiles and Functional Prediction of Circular RNAs and Long Non-coding RNAs in the Hippocampus of Nrf2-Knockout Mice

doi: 10.3389/fnmol.2019.00196

Figure Lengend Snippet: The primers used in qRT-PCR experiments.

Article Snippet: Hybridization solution (50 μl) was dispensed into the gasket slide (Agilent, Cat No. G2534-60003, United States) and assembled to a mouse circRNA microarray (8 × 15K, Arraystar) slide.

Techniques:

Journal: Frontiers in Molecular Neuroscience

Article Title: Differential Expression Profiles and Functional Prediction of Circular RNAs and Long Non-coding RNAs in the Hippocampus of Nrf2-Knockout Mice

doi: 10.3389/fnmol.2019.00196

Figure Lengend Snippet: Top 10 up- and down-regulated DEcircRNAs in the hippocampus of Nrf2 (−/−) mice.

Article Snippet: Hybridization solution (50 μl) was dispensed into the gasket slide (Agilent, Cat No. G2534-60003, United States) and assembled to a mouse circRNA microarray (8 × 15K, Arraystar) slide.

Techniques:

Journal: Frontiers in Molecular Neuroscience

Article Title: Differential Expression Profiles and Functional Prediction of Circular RNAs and Long Non-coding RNAs in the Hippocampus of Nrf2-Knockout Mice

doi: 10.3389/fnmol.2019.00196

Figure Lengend Snippet: QRT-PCR validation of the expression levels of candidate circRNAs (A) and lncRNAs (B) . * p < 0.05 and ∗∗ p < 0.01. The deep red column indicates the expression status of lncRNAs through microarray analyses; the blue column indicates the expression status of lncRNAs through qRT-PCR experiments. n = 3.

Article Snippet: Hybridization solution (50 μl) was dispensed into the gasket slide (Agilent, Cat No. G2534-60003, United States) and assembled to a mouse circRNA microarray (8 × 15K, Arraystar) slide.

Techniques: Quantitative RT-PCR, Biomarker Discovery, Expressing, Microarray

Journal: Frontiers in Molecular Neuroscience

Article Title: Differential Expression Profiles and Functional Prediction of Circular RNAs and Long Non-coding RNAs in the Hippocampus of Nrf2-Knockout Mice

doi: 10.3389/fnmol.2019.00196

Figure Lengend Snippet: DEcircRNA-miRNA-DEceRNA interaction subnetworks of up-regulated circRNAs and down-regulated circRNAs in the Nrf2 (–/–) hippocampus. (A) Subnetwork of mmu_circRNA_44531 in the Nrf2 (–/–) hippocampus. (B) Subnetwork of mmu_circRNA_34132 in the Nrf2 (–/–) hippocampus. (C) Subnetwork of mmu_circRNA_000903 in the Nrf2 (–/–) hippocampus. (D) Subnetwork of mmu_circRNA_018676 in the Nrf2 (–/–) hippocampus. (E) Subnetwork of mmu_circRNA_45901 in the Nrf2 (–/–) hippocampus. (F) Subnetwork of mmu_circRNA_33836 in the Nrf2 (–/–) hippocampus. (G) Subnetwork of mmu_circRNA_34137 in the Nrf2 (–/–) hippocampus. (H) Subnetwork of mmu_circRNA_34106 in the Nrf2 (–/–) hippocampus. (I) Subnetwork of mmu_circRNA_008691 in the Nrf2 (–/–) hippocampus. (J) Subnetwork of mmu_circRNA_003237 in the Nrf2 (–/–) hippocampus. Yellow nodes indicate DEcircRNAs. Magenta and green nodes indicate miRNAs sponged by DEcircRNAs and the gene ID of their DEceRNAs, respectively. Edges represent interactions.

Article Snippet: Hybridization solution (50 μl) was dispensed into the gasket slide (Agilent, Cat No. G2534-60003, United States) and assembled to a mouse circRNA microarray (8 × 15K, Arraystar) slide.

Techniques: